Ready to use kit for glucose

University of Wisconsin-Madison
Department of Food Science
Research Assistant

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cayman Chemical

Product Name:

Glucose Colorimetric Assay Kit

Catalog Number:

10009582

I am looking at the effect of eating yogurt before a meal on postprandial blood glucose change. Due to the large quantity of my samples, I chose the microplate method. This kit comes with all the reagent needed and even the microplates.

Experimental Design and Results Summary

Application

To measure human plasma glucose level

Starting Material

Human NaHep plasma (also works on EDTA plasma)

Protocol Overview

The procedures are pretty simple and straight forward. 1) Thaw the samples and bring the kit to room temperature. 2) Prepare the standard stock solution and series dilutions. 3) Dilute samples with the provided buffer. 4) Add assay buffer to the plate 5)Load diluted samples, standards, control to the plate----add enzyme mixture---incubate--read.

Tips

Make sure to do the initial 10 times dilution for the highest standard solutuion

Results Summary

Works very well.

Additional Notes

None

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Summary

The Good

Easy, everything included

The Bad

More dilution buffer would be helpful

The Bottom Line

I will use this kit again.

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