Abcam
Anti-p16 ARC antibody [EP1551Y]
ab51243
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We are evaluating the basal level of DNA damage markers in wild type and clock deficient mutant mice to understand their role in specific pathological processes. We would like to compare stress-induced senescence in vivo in mouse lung samples and p16 can be used as a marker for senescence in vivo in mouse models.
Western Blot
Mouse lung homogenates
Overnight at 4 degrees C (1:1000 dilution)
5% milk at room temperature for 1 hour
1 hour at room temperature
NA
ECL based detecion using Bio-rad chemidoc MP
Very specific (~16 kDa) target band observed via immunoblotting.
None
Works well for Western blot of mouse samples
Didn't note any
Very good antibody for WB that can be used for mouse samples.