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HSP-60 antibody staining on stem cells
(1)
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November 17, 2014
The antibody was used to detect the effect of environmental toxicants on mitochondrial unfolded protein response. Specifically, this ...
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Phospho Akt protein expression analysis via Western blotting
(1)
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Cell Signaling Technology
November 14, 2014
Phospho Akt/PKB protein expression was characterized via Western blot using the Phospho-Akt (Ser473) Mouse mAb (1:1000).
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Excellent Ab for intracelluar staining of mouse IL-10
(1)
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BioLegend
November 14, 2014
We are looking at the intracellular staining of mouse IL-10 in mouse CD4 T cells from the spleen when stimulated by PMA, Inomycin and BFA. ...
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Akt/PKB protein expression analysis via Western blotting
(1)
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Cell Signaling Technology
November 13, 2014
Akt/PKB protein expression was analyzed via Western blotting with Akt Mouse mAb (1:1000) in murine cardiomyocytes
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ChIP grade HIF1-Alpha
(1)
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Thermo Fisher Scientific
November 13, 2014
HIF1-Alpha is a trancriptional factor which is expressed upon deprivation of oxygen concentration and regulates several downstream targets. ...
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Mouse specific cleaved PARP (cell death marker)
(1)
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November 12, 2014
Programed cell death (PCD) is a critical process that underlies normal tissues development and serves as a homeostatic mechanism to maintain...
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Cleaved caspase-3 Antibody for WB
(1)
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Cell Signaling Technology
November 12, 2014
Programed cell death (PCD) is a critical process that underlies normal tissue development and serves as a homeostatic mechanism to maintain ...
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Great Antibody for FLAG immunoprecipitations
(1)
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November 11, 2014
Our lab uses Anti-FLAG® M2 Magnetic Beads (produced in mouse, Sigma) for protein interaction co-immunoprecipitations. Invariably, some of ...
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Clear and Crisp DnaK Antibody
(1)
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Abcam
November 11, 2014
We generally use DnaK protein levels as a cytosolic control for western blotting. This allows us to determine if general protein levels are ...
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Western blotting via anti-Bcl-x
(1)
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November 10, 2014
Anti-Bcl-x protein expression was characterized via western blot by using purified rabbit Anti-Bcl-x (1:500)
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MCP-1 detection in A549 cells
(1)
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Santa Cruz Biotechnology, Inc.
November 10, 2014
MCP-1 protein expression was characterized via Western blot by using purified MCP-1 (C-17) Antibody
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GAPDH protein expression analysis via Western blotting
(1)
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MilliporeSigma
November 07, 2014
GAPDH protein expression was characterized via Western blot using the anti-GAPDH antibody (1:10000).
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Western blot via p44/42 MAPK (Erk1/2) Mouse mAb from Cell Signaling Technology
(1)
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Cell Signaling Technology
November 07, 2014
Erk1/2 protein expression was characterized via Western blot in HL-1 cells
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Excellent Ab for intracellular staining of mouse IFN-g
(1)
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BioLegend
November 06, 2014
We are looking at the intracellular staining of mouse IFN-gamma in CD4 memory T cells when stimulated by PMA, Ionomycin and BFA.
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CD3 Antibody works at high concentration
(1)
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BioLegend
November 06, 2014
We are trying to sort CD3 population in mouse spleen cells by Brilliant Violet 650™ anti-mouse CD3 Antibody.
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SuperBlock Really is Super
(1)
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November 05, 2014
I have been working with SuperBlock as a blocking buffer for the last three years and it has dramatically improved the quality of my western...
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QIAxcel for Saving Time
(1)
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QIAGEN - Sample to Insight
November 05, 2014
Our lab studies a specific subset of B cells. Our research focuses on determining the overall function of these B cells in both humans and ...
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A great DNA ladder
(1)
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November 04, 2014
We run a lot of 1-2% agarose gels to test the size of our sample DNA, especially PCR products. We have used several types of ladders in the ...
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Primer is superier to other version of ECL
(1)
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November 04, 2014
I was trying to get a Western blot done for tissue expression of the trans gene from transgenic mice we created. I was having trouble with ...
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PCR amplification using REDTaq® ReadyMix™ PCR Reaction Mix
(1)
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November 03, 2014
I have used the REDTaq ReadyMix PCR reaction mix to amplify mutation containing regions of DNA for downstream Sanger sequencing.
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Very good CD304 antibody
(1)
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BioLegend
November 03, 2014
We use this antibody to discriminate between thymus derived versus peripheral induced regulatory T cells in mouse spleen on a regular basis....
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Mouse TNF-alpha ELISA MAX™ Standard
(1)
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BioLegend
October 31, 2014
Detect TNF-a production in LPS activated macrophage culture. It is an easy way to set up this traditional measure system.
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Evaluating CD62E expression by IF
(1)
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October 31, 2014
R and D systems mouse anti-human CD62E was tested on 293T human cells ectopically expressing human CD62E by immunoflourescence. Fixation and...
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Quickly assess cell death- Caspase 3/7 activation
(1)
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Promega
October 30, 2014
An easy to way to measure cell death in a plate- based assay. This kit allows the scientist to rapidly measure the levels of caspase-3/7 ...
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Phospho-ERK1/2 detection in HL-1 via Western blotting
(1)
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Cell Signaling Technology
October 30, 2014
Phospho-ERK1/2 protein expression was characterized via western blot in HL-1 cells
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Good enzyme, some optimization likely required
(1)
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New England Biolabs
October 29, 2014
A loop-mediated isothermal amplification (LAMP) study was conducted to detect the presence of pathogen DNA in food samples. Bst 2.0 from NEB...
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Rabbit anti-Hsp60 Ab for IF
(1)
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GeneTex
October 29, 2014
Immunofluorescence in MEFs staining for Hsp60. Hsp60 was chosen as a mitochondrial marker to examine the mitochondrial network in MEFs.
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Mouse IFN-gamma ELISA MAX™ Standard
(1)
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BioLegend
October 28, 2014
To measure the IFN-gamma production in conA activated T cell culture. To set up a measurement system for T cell activation.
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FGF2 protein expression via western blotting
(1)
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MilliporeSigma
October 27, 2014
FGF2 protein expression was characterized via western blot using the Anti-FGF-2/bFGF Antibody (1:500) in murine HL-1 cells.
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Great product for quick and easy miRNA extraction
(1)
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Thermo Fisher Scientific
October 24, 2014
The goal of the experiment was to examine microRNA and gene expression changes in the hypothalami of rats at Postnatal day 15, P30, P45, and...
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Excellent mouse IL-7 cytokine
(1)
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BioLegend
October 23, 2014
Basically, we are culturing one mouse B cell line which needs IL-7 for survival and proliferation. At the beginning, we used 10ng/ml, and ...
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Sigma FLAG Antibody
(1)
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October 22, 2014
We used this antibody for Western blotting of the FLAG-tagged proteins expressed in mammalian cell lines. Works well if stored properly. ...
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Highly recommend LA Taq Polymerase
(1)
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October 21, 2014
This taq is optimum to expand any gene from 1kb to 15kb. It works specifically great for the ones bigger than 2kb. The PCR product can also ...
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Western blotting: human CXCR4 (Fusin) MAb (Clone 12G5)
(1)
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R&D Systems, a Bio-Techne Brand
October 20, 2014
Western blotting was performed using anti-human CXCR4 (Fusin) antibody in humen endothelial cells
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Workhorse
(1)
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October 17, 2014
Our lab studies a specific subset of B cells. Our research focuses on determining the overall function of these B cells in both human and ...
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Beta-Actin protein expression analysis via Western blotting
(1)
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Santa Cruz Biotechnology, Inc.
October 16, 2014
Beta-Actin protein expression was characterized via Western blot using the anti-ß-Actin (1:10000) from Santa Cruz.
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AquaLab equipment works well
(1)
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October 15, 2014
We measure water activity for a number of foods. The AquaLab Water Activity Meter is very efficient at conducting these measurements and is ...
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SF2/ASF protein expression analyzes via Western blot
(1)
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Thermo Fisher Scientific
October 14, 2014
SF2/ASF protein expression was characterized via western blot using the SF2/ASF Antibody (dilution 1:1000).
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KAT6A antibody evaluated for Western blot and pull down experiment
(1)
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October 13, 2014
In my experiment we study protein-protein interaction between KAT6a and a KAT6a interacting protein. The antibody gave very nice pull-down ...
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Simple Diagnostic Test for STEC
(1)
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October 10, 2014
Our lab is in the pathogen detection field, we tested the The Verotoxin-producing Escherichia coli Detection Kit from SA Scientific to test ...
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Great primers at good prices
(1)
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October 09, 2014
I have had a great experience ordering primers from IDT. They have always given me high-quality primers, shipped very quickly, and their ...
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Thermo Scientific Precision Digital Water Bath, with Lab Armor Beads
(1)
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October 08, 2014
Using cell culture, our lab assesses the viability of mouse cortical cells through several assays, including radioactive uptake. Since our ...
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Solid workhorse machine
(1)
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Thermo Fisher Scientific
October 07, 2014
We wanted to perform qPCR testing to determine if treatment of a G-protein-coupled receptor with an agonist would affect the transcription ...
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Excellent antibody for immunofluoresence detection of YAP/TAZ in mouse cells
(1)
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Cell Signaling Technology
October 02, 2014
The goal of the study was to detect YAP/TAZ localization in mouse embryonic fibroblasts by immunofluorescence
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Very good antibody for detection of SHP2 by immunofluoresence
(1)
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October 01, 2014
The goal of this experiment was to determine the cellular localization of SHP2 in mouse embryonic fibroblasts (MEFs)
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BACH2 copy number analysis using Life Technologies TaqMan Assays
(1)
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September 30, 2014
I am interested in copy number variations of particular genes in B-cell lymphoma. I have used the Life Technologies Copy Number Assays to ...
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PE/Dazzle™ 594 anti-human CD19 Antibody
(1)
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BioLegend
September 26, 2014
Cells Depot™ preserved human PBMCs was stained with PE/Dazzle™ 594 anti-human CD19 Antibody diluted in flow cytometry buffer (PBS + 2% CS). ...
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Use of Extan PMP22 Kit by Centogene
(1)
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September 19, 2014
Extan Kit for PMP22 by Centogene was used to identify point mutations responsible for CMT1A / CMT1E. The instructions in the kit for the ...
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Quantifying the Terminal Complement Complex (C5b-9) in Plasma
(1)
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September 08, 2014
Activation of any of the three complement pathways (classical, alternative, or lectin) results in the formation of the terminal complement ...
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PPi Release Assay Works Well
(1)
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September 08, 2014
I used the Lonza PPi release assay to measure the activity of a pyrophosphorylase. I also compared the activity to a suspect ...
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