Very Good Anti-Human CD45 antibody

Dept of biomedical Sciences
TTUHSC El Paso
Post doc

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BD Biosciences

Product Name:

CD45 PE Antibody

Catalog Number:

555483

Browse Similar Products: CD45 Antibodies

Single cell suspensions prepared from the spleen of human PBMC reconstituted mice was used for flow cytometric analysis. I have used this antibody to separate human cells from the spleen of these humanized mice. The cells were stained with this CD45 antibody and analyzed by flow cytometry. The staining looks pretty good with two separate populations of CD45+ and CD45- cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Splenocytes from humanized mice

Primary Incubation

30 min

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry on PE Channel

Results Summary

Clear CD45 positive and negative population which confirm the specificity of this antibody

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Great specificity

The Bad

Titration is required

The Bottom Line

Very good antibody for detecting human CD45 expression

Share your experience with other scientists. Write a Review! »

Join the discussion