Ku70 antibody for mouse lung samples

April 20, 2015

BlueskyReddit
Environ. Med.
University of Rochester
Postdoc

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Abcam

Product Name:

Anti-Ku70 antibody

Catalog Number:

ab3114

Info: View Product View Product Specs

Browse Similar Products: Ku70 Antibodies

We are evaluating the basal level of DNA damage markers in wild type and clock deficient mutant mice to understand their role in specific pathological processes. We used this Ku70 antibody to detect the basal level of Ku70 in different transgenic and mutant mouse lung samples.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse lung homogenates

Primary Incubation

1:1000 dilution Ab in 5% milk overnight at 4 degrees C

Blocking Agent

5% milk at RT for an hour

Secondary Incubation

1:20,000 secondary antibody in 5% BSA at RT for 1 hour.

Tertiary Incubation

NA

Detection

ECL based detection using Bio-rad chemidoc MP

Results Summary

We found this antibody to work with mouse lung samples with less nonspecific bands.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Ku70 antibody good for WB analysis in mouse samples

The Bad

The band that it detects is slightly lower than expected

The Bottom Line

Works well for mouse samples with some additonal nonspecific bands below the target band (lower than expected size).

Share your experience with other scientists. Write a Review! »

Join the discussion