Thermo Scientific Pierce
ZO-1 / TJP1 Antibody
61-7300
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In our laboratory we have to track airway epithelial cells throughout differentiation. Tight junction formation is among those steps.
Immunofluorescence
NHBE
1:500 in 3% BSA 1 hour or overnight, 4 degrees
3% BSA
1:500 donkey anti-rabbit AF 555 (Life technologies) 30-60 minutes in the dark
NA
Confocal microscopy
I would recommend to always pre-extract the cells on ice for 2 minutes with an ice-cold buffer containing 0.2% Triton X-100 before fixation. This is when we achieve most consistent results
None
As soon as we optimized we got great ZO-1 staining
Pricey and sometimes it requires pre-extraction before fixation
Great product