Good for Duplex PCR

University of Miami
Cell Biology
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Bio-Rad

Product Name:

SsoAdvanced™ Universal Probes Supermix

Catalog Number:

172-5280

Info: Supplier Page 

I use this supermix when performing duplex Taqman probe PCR. It amplifies both probes and saves time and sample because I can do duplexing with it.

Experimental Design and Results Summary

Application

PCR

Starting Material

Various

Protocol Overview

Mix 2X Ssoadvanced mix with Taqman probes and water to make a master mix. Add master mix to wells and then add the sample. Run PCR

Tips

Be sure the Ssoadvanced is well mixed before making master mix

Results Summary

The data show a sample with 2 different color Taqman probes. The Ssoadvanced amplified both samples.

Additional Notes

None

Related Categories

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Summary

The Good

Easy to use

The Bad

None

The Bottom Line

This is a good product and I would recommend it.

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