Life Technologies
Chicken anti-Mouse IgG (H+L) Secondary Antibody
A-21463
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Primary mouse antibody against acetylated tubulin was detected. It gives a strong signal easily detectable in confocal microscopy and low background.
Immunofluorescence
Primary Human Epithelial Cells
Primary anti-mouse antibody, overnight, 4 degrees
3% BSA
Chicken anti-Mouse AlexaFluor 647, 1:500, 1 hour at room temperature
NA
Confocal Fluorescent Microscopy
Image shows AlexaFluor 647 pseudo-colored white staining ciliated epithelial cells. The primary antibody was anti-acetylated tubulin, so 647 should be staining cilia. This show the secondary binds to the primary and the lack of strong background signal shows it has nice specificity.
None
Binds to primary antibody with low background
This is a good secondary antibody and I would recommend to a colleague.