MUC5AC Immunfluorescence Staining in NHBE

Cell Biology
University of Miami
RA3

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Quality of Results

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Company:

Santa Cruz Biotechnology, Inc.

Product Name:

Mucin 5AC (H-160) Antibody

Catalog Number:

sc-20118

Info: View Product View Product Specs

Browse Similar Products: Mucin 5AC Antibodies

We work with primary cells (airway epithelial cells) in our lab and can use several different donkey alexas with different fluorophore conjugations in a single staining without any issue. This antibody was purchased to use as a marker of goblet cells in airway epithelial cells.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

NHBE

Primary Incubation

1:500 in 3% BSA overnight in the cold

Blocking Agent

3% BSA

Secondary Incubation

Donkey anti rabbit AF555 1:2000 in 3% BSA 30-60 min RT in the dark

Tertiary Incubation

DAPI

Detection

Confocal microscopy 63X

Results Summary

Great IF results using this SC antibody

Additional Notes

None

Related Categories

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Summary

The Good

Works great for IF

The Bad

A little expensive but the vial lasts forever since the dilution we use is very high.

The Bottom Line

Great product

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