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Date Published
Fixable Viability Dye eFluor® 660
(1)
Write a Review
December 16, 2014
Basically, this dye was used to stain dead CD4 T cells, which can be detected by APC channel.
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Phospho-S6 Ribosomal Protein (Ser235/236) Antibody (for Western Blot) from Cell Signaling Technology
(1)
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Cell Signaling Technology
December 16, 2014
This antibody was used in a study that investigated the role of Phospho- S6 Ribosomal Protein (Ser235/236) in fibroblast cells from a ...
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I love BioLegend Brilliant Violet 421, perfect replacement for PB
(1)
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BioLegend
December 15, 2014
Pacific Blue is good because it doesn't have compensation with most used colors like PE, APC, APC-CY7, PE-CY7, and FITC. However, PB is not ...
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Quick Latex Agglutination Test Kit
(1)
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Abraxis LLC
December 15, 2014
The E. coli O103 Latex Test Kit was used as part of an E. coli screening protocol to determine the presence of the top seven shiga toxin ...
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NBS1 A301-290A works great for WB and IP
(1)
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Bethyl Laboratories, Inc.
December 15, 2014
The aim was to test whether depletion of the SUMO pathway disrupts the associations of NBS1 and its binding proteins such as ATM and MRE11. ...
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Excellent loading control
(1)
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Santa Cruz Biotechnology, Inc.
December 12, 2014
Most of the antibodies are normalized with respect to GAPDH. This antibody is very specific and particularly inexpensive. It works both with...
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Easy to use, reliable rodent Estradiol assay kit
(1)
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Calbiochem
December 12, 2014
Calbiochem has a very reliable line of ELISA kits for rodents. Their Estradiol (E2) is designed to assay the most potent of the circulating ...
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Review of Mouse IFN gamma ELISPOT kit
(1)
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December 12, 2014
The aim of the research is to monitor IFN gamma production in mouse splenocytes in response to vaccination with Newcastle disease virus ...
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Small, easy and fast basic cell counter
(1)
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Bio-Rad
December 11, 2014
I bought this cell counter after researching for an automated cell counters. This has a smaller footprint and is fairly easy to use. My ...
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BUV395 amazing addition in the multicolor panel
(1)
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BD Biosciences
December 11, 2014
563793 and 563557 BUV provides a new addition in the multi-color flowcytometry panel. I have tried both Anti-mouse CD19 and CD45R (B220)...
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Cleaved PARP antibody
(1)
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Cell Signaling Technology
December 11, 2014
Poly(ADP-ribose) polymerase (PARP) is a nuclear DNA binding protein which can detect DNA strand breaks and is involved in base excision ...
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Excellent ab for iTreg population staining
(1)
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December 10, 2014
This product was used for experiments running phenotypic detection of CD4 T cells and their roles in a mouse colitis model. This Ab was ...
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Alpha-actinin antibody for cardiomyocyte staining
(1)
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December 10, 2014
Excellent antibody to characterize human iPSC-derived cardiomyocytes in cell culture.
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MRPS29 antibody
(1)
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December 09, 2014
Mitochondrial ribosomal protein S29 (MRPS29) is a mitochondrial pro-apoptotic protein also known as death associated protein 3 (DAP3). It ...
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Great ATM antibody for both WB and IP
(1)
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Bethyl Laboratories, Inc.
December 09, 2014
ATM is a major transducer kinase that regulates DNA damage response and cell cycle checkpoint. Interaction of ATM and MRN complex (MRE11-...
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Human IFN alpha ELISA
(1)
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Mabtech AB
December 09, 2014
Many cytokines are elevated in acute dengue infection and the main aim was to determine if severe dengue could occur due to reduction in ...
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Excellent Ab for IL-4 intracellular staining
(1)
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BioLegend
December 08, 2014
This Ab was used to detect inter-cellular staining of IL4 from mouse CD4 T cell differentiated Th2 cells in vitro.
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Cardiac troponin T immunofluorescence of human cardiomyocytes
(1)
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Thermo Fisher Scientific
December 08, 2014
I used this antibody to characterize human iPSC-derived cardiomyocytes in culture using immunofluorescence. I selected this antibody as it ...
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Excellent ab for iTreg population staining
(1)
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BioLegend
December 08, 2014
This Ab was used to stain mouse CD4 T cells differentiated iTreg in vitro including CD4+CD25+Foxp3+.
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Great Phospho-Chk1 antibody
(1)
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Cell Signaling Technology
December 05, 2014
Phosphorylation of Chk1 at S317 is a hallmark of the activation of cell cycle checkpoint. The goal of this experiment is to examine whether ...
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Ab used for staining of mouse naive T cells from spleen
(1)
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BD Biosciences
December 05, 2014
Here I am looking at two parameters at the same time (CD25 PE X-axis and Foxp3e450 Y-Axis). This ab was used to stain mouse naive CD4 T ...
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Good early stem cell marker
(1)
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MilliporeSigma
December 05, 2014
These antibodies were used in work in my previous lab with Dr. Scott Whittemore at the Kentucky Spinal Cord Injury Research Center at the ...
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Excellent ab for CD4 population staining
(1)
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BioLegend
December 04, 2014
This product was used for experiments running phenotypic detection of CD4 T cells and their roles in a mouse colitis model. This mouse CD4 ...
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Very reliable alpha-tubulin antibody
(1)
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Abcam
December 04, 2014
Tubulin is used as a loading control at 50KDa for antibodies that are lower than 40KDa. Most other loading markers fall under 40Kda which ...
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HSP60 antibody
(1)
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December 04, 2014
HSP60 has been used as a loading control for mitochondrial protein. It showed the same level of actin as another loading control for ...
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MIF quantitative ELISA
(1)
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BioLegend
December 03, 2014
MIF levels have been shown to be high in patients with acute dengue. Therefore, we set out to validate if this can be used as a biomarker to...
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Excellent Ab for IL-17 intracellular staining
(1)
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BioLegend
December 02, 2014
This Ab was used to detect intra-cellular staining of IL17 from mouse CD4 T cell differentiated pathogenic Th17 cells in vitro.
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Excellent ab for CD44 memory T cell population staining
(1)
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BioLegend
December 02, 2014
This product was used for experiments running phenotypic detection of CD4 T cells and their roles in a mouse colitis model. This ab can be ...
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FFPE Immunohistochemistry of C5b-9
(1)
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Abcam
December 02, 2014
Activation of the complement system ultimately leads to the formation of the membrane attack complex, or MAC. The MAC is composed of the ...
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An alternative anti-EpCAM antibody
(1)
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December 01, 2014
EpCAM is a marker used very often in research to detect epithelial cells. This is very important in mixed populations (e.g. tumors ...
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Imaging CD14 positive human monocytes in peripheral blood mononuclear cells (PBMC) using wide-field microscope
(1)
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Abcam
December 01, 2014
We isolated peripheral blood mononuclear cells from fresh human blood using Ficoll Paque Plus protocol and stained them with anti CD14-FITC ...
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Purification of PCR products for cloning
(1)
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Promega
December 01, 2014
I routinely cloning PCR products to various plasmids for expression and conditional knock down experiments. I need high purity PCR product ...
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Failed to improve GFP signal
(1)
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MilliporeSigma
November 26, 2014
Embryonic rat dorsal root ganglion neurons were infected with a GFP-tagged lentivirus in culture. The signal from the virus was present, ...
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Mitochondrial marker for WB- AIF antibody
(1)
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Santa Cruz Biotechnology, Inc.
November 26, 2014
HEK293 cells were fractionated into cytosol and heavy membrane fractions. AIF was utilized to track the purity of the fractionation as AIF...
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Loading Control: Tubulin (a must have for WB)
(1)
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November 26, 2014
Western blot detection usually requires the use of loading controls to interpret the results of experiments. Tubulin is ubiquitously ...
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Acetylated tubulin Ab
(1)
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November 25, 2014
I have used this on non-model organisms for staining neurons and worked great!
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S100b
(1)
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Abcam
November 25, 2014
These antibodies were used in work in my previous lab with Dr. Scott Whittemore at the Kentucky Spinal Cord Injury Research Center at the ...
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Itgav protein expression analysis via Western blotting
(1)
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Abcam
November 24, 2014
Itgav protein expression was characterized via Western blot using the Integrin alpha V antibody (1:500).
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Very potent antibody for pERK flow staining
(1)
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Cell Signaling Technology
November 24, 2014
Upon T-cell receptor activation, T cells turn on MAPK pathways including the ERK (p44/42) activation, which is critical for T cell ...
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VEGF protein expression - Western blotting
(1)
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November 24, 2014
VEGF protein expression was characterized via Western blotting using the VEGFA polyclonal antibody (1:1000) from Abnova
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Staining for Mfn 2 in neural stem cells
(1)
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Abcam
November 21, 2014
Anti Mfn-2 was used to detect effect of environmental toxicants on mitochondrial fusion in stem cells. This antibody worked well and the ...
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P38 protein expression was characterized via Western blotting using the Anti-p38 MAP Kinase (341-360) Rabbit pAb
(1)
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MilliporeSigma
November 21, 2014
P38 protein expression was characterized via Western blotting using the Anti-p38 MAP Kinase (341-360) Rabbit pAb (1:1000) in HL-1 cells
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Phospho-p38 - Western Blot
(1)
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Cell Signaling Technology
November 21, 2014
phospho- p38 protein expression was characterized via Western blotting using the Phospho-p38 MAPK (Thr180/Tyr182) Antibody from Cell ...
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Fluorescent Detection Reagent Eases LAMP Detection
(1)
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November 20, 2014
Our lab was conducting Loop-mediated isothermal amplification (LAMP) experiments using both SA scientific kits and bst 2.0 homemade ...
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Cell screening kit: Easy to use, great data
(1)
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BioLegend
November 20, 2014
I found the Biolegend LEGENDScreen incredibly easy to use and following the manufacturer's instructions produced great data to help me ...
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Beautiful neuronal staining
(1)
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Bio-Rad
November 19, 2014
These antibodies were used in work in my previous lab with Dr. Scott Whittemore at the Kentucky Spinal Cord Injury Research Center at the ...
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Nice Astrocyte Marker
(1)
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Abcam
November 19, 2014
These antibodies were used in work in my previous lab with Dr. Scott Whittemore at the Kentucky Spinal Cord Injury Research Center at the ...
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SC35 protein expression analysis via Western blotting
(1)
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Abcam
November 18, 2014
SC35 protein expression was characterized via western blot using the SC35 Antibody (1:1000)
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Lipid ELISA
(1)
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November 18, 2014
Our lab studies a specific subset of B cells. Our research focuses on determining the overall function of these B cells in both human and ...
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Anti HSP-10 staining on stem cells
(1)
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Santa Cruz Biotechnology, Inc.
November 17, 2014
The antibody was used to detect the effect of environmental toxicants on mitochondrial unfolded protein response. Specifically, I used this...
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