Life Technologies
Donkey anti-Mouse IgG (H+L) Secondary Antibody
A-31570
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I was using the secondary antibody to detect a primary HA antibody that was derived from a mouse. The HA should have been nuclear, which is exactly where I found secondary antibody fluorescence. This is a good secondary antibody that detects primary mouse antibodies and gives strong signal for fluorescent microscopy.
Immunofluorescence
Primary Human Epithelial Cells
Primary anti-mouse antibody, overnight, 4 degrees
3% BSA
Donkey anti-Mouse AlexaFluor 555, 1:500, 1 hour at room temperature
NA
Confocal Fluorescent Microscopy
Detected strong 555 signal in primary human epithelial cells stained with a primary mouse antibody against a nuclear localized protein. 555 signal co-localized with nuclear staining indicating it had bound to the primary antibody. There was little non-nuclear signal indicating good specificity.
None
Binds to primary antibody with low background
This is a quality secondary and I would recommend.