Abcam
Anti-BMAL1 antibody
ab93806
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We are evaluating the changes in clock proteins in wild type and clock deficient mutant mice to understand their role in specific pathological processes. We used this antibody to determine levels of Bmal1 in different transgenic/mutant mouse lungs.
Western blot
Mouse lung homogenates
Overnight at 4 degree (1:1000 dilution) in 5% milk with less nonspecific bands or 5% BSA with additional nonspecific bands
5% milk
1:20,000 secondary Ab at RT
NA
ECL based detection using Bio-rad chemidoc MP
Detects specific Bmal1 target band by immunoblotting.
None
Excellent abtibody for Western blot analysis
Not noted.
Works well for immunoblotting analysis of mouse samples