NR1D1 Antibody used for Western blotting

Environ. Med.
University of Rochester
Postdoc

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Company:

Novus Biologicals

Product Name:

NR1D1

Catalog Number:

H00009572-M02

Info: View Product View Product Specs

Browse Similar Products: NR1D1 Antibodies

We are evaluating changes in clock proteins in wild type and clock deficient mutant mice to understand their role in specific pathological processes. This product was used to determine levels of NR1D1 in mouse lung samples.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Lung homogenates

Primary Incubation

1:1000

Blocking Agent

5% Milk

Secondary Incubation

1 hour

Tertiary Incubation

NA

Detection

ECL

Results Summary

Nonspecific bands were there, but the target band is also present as mentioned in the data sheet

Additional Notes

None

Related Categories

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Summary

The Good

The antibody detects the target

The Bad

Too many nonsepcific bands develop along with the target band

The Bottom Line

This antibody is not good for Western blot of the target Nr1d1

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