Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Environ. Med.
University of Rochester
Postdoc

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Company:

Abcam

Product Name:

Anti-BMAL1 antibody

Catalog Number:

ab93806

Info: View Product View Product Specs

Browse Similar Products: BMAL1 Antibodies

We are evaluating the changes in clock proteins in wild type and clock deficient mutant mice to understand their role in specific pathological processes. Bmal1 antibody from Abcam works well for immunohistochemistry in mouse lungs (Formalin/PFA-fixed paraffin-embedded sections).

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse lung section paraffin embedded

Primary Incubation

1:250 at 4 degrees overnight

Blocking Agent

10% donkey serum for 1 hour at RT

Secondary Incubation

Fluorescent conjugated secondary antibody at 1:1000 dilution at RT

Tertiary Incubation

NA

Detection

Fluorescence microscopy

Results Summary

Bmal1 antibody works in formalin-fixed mouse lung tissues. We very able to clearly detect Bmal1 expression in lung ATII cells and bronchial epithelial cells.

Additional Notes

None

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Summary

The Good

Excellent antibody available on the market for mouse Bmal1 target (IF)

The Bad

Noted none

The Bottom Line

Recommend this antibody for IF in mouse samples.

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