Immunofluorescence staining using Life Technologies AF 2nd Ab

Cell Biology
University of Miami
RA3

Overall

Quality of Results

Ease-of-Optimization

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Company:

Life Technologies

Product Name:

Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor® 647 conjugate

Catalog Number:

A-21235

We study primary cells (airway epithelial cells) in our lab and do a lot of confocal microscopy. Life Technologies is one of our favorite companies to get secondary antibodies from.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

NHBE

Primary Incubation

Any mouse antibody

Blocking Agent

3% BSA or 5 % milk

Secondary Incubation

1:2000 goat anti-mouse AF647 (Life Tech)

Tertiary Incubation

NA

Detection

Confocal microscopy

Results Summary

Secondary AF-conjugated antibodies from Life technologies have never failed us

Additional Notes

Image shows ecadherin staining using this secondary antibody

Image Gallery

Summary

The Good

Easy to optimize and lasts forever

The Bad

They tend to aggregate when they get older

The Bottom Line

Great product, great price, therefore great images

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