Akt1 Antibody from Cell Signaling

November 12, 2015

BlueskyReddit
Institute of Genetic Medicine
Johns Hopkins University
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Akt1 Antibody

Catalog Number:

2938

This antibody was used to detect total Akt1 expression and used as a control for phosphorylated Akt S473.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human HNSCC cell line whole cell lysate

Primary Incubation

1:2000, 2 hours at RT

Blocking Agent

5% milk

Secondary Incubation

1:2000, 1 hour at RT

Tertiary Incubation

NA

Detection

Enhanced Chemiluminescence Detection Kit

Results Summary

This antibody perfectly detected total Akt expression levels by western blot in human HNSCC cells.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Detects a specific band and the signal is strong

The Bad

None

The Bottom Line

This is a perfect antibody for western blot.

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