Cell Signaling Technology
Bcl-2 (50E3) Rabbit mAb
2870
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I checked BCL2 levels upon oxidative stress in 293T cells. Beta actin was used as control. BCL2 is one of the most well-studied anti-apoptotic molecules and my research mainly focuses on apoptosis.
Western Blot
293T whole cell lysate
Anti-BCl2 antibody diluted in 5% BSA in TBST, incubated O/N at 4 degrees
5% non fat milk for an hour at RT
Anti-rabbit secondary antibody at 1:3000 dilution in blocking solution, incubated at room temperature for an hour
NA
ECL
I got specific bands for BCL2 upon giving oxidation stress to 293T cells.
None
Specificity
Nice antibody for western blotting