Human TIMP-1 ELISA Kit

RUSH UNIVERSITY MEDICAL CENTER
ORTHOPAEDIC SURGERY
POSTDOC

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Company:

Invitrogen

Product Name:

Human TIMP-1 ELISA kit

Catalog Number:

KHC1491

This kit is for the detection of human TIMP-1 protein concentration ELISA kit. It's provided with every reagent needed and a pre-coated plate. Standard curve range is from 1.56ng/ml to 25ng/ml, which is relatively wide and good. Works well in detecting the TIMP-1 protein concentration of the human cultured annulus fibrosus cell culture media.

Experimental Design and Results Summary

Application

ELISA

Starting Material

None

Protocol Overview

Add in 50ul of sample followed by 50ul of biotinylated antibody for 2h at RT and wash. Add in Streptavidin-HRP and wash. Add in substrate solution and finally stop solution.

Tips

Warm up the reagents to room temperature before use

Results Summary

This kit works well in detecting the TIMP-1 concentration in the cell culture media from human annulus fibrosus cell.

Additional Notes

None

Image Gallery

Summary

The Good

Provide precoated plate and is very convenient.

The Bad

Price is relatively high.

The Bottom Line

This kit works well in detecting the TIMP-1 protein concentraion from human cell culture media.

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