Abcam
Anti-KDEL antibody
ab12223
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Browse Similar Products: KDEL Western blot Antibodies
This antibody was used to verify fraction purity in a subcellular fractionation of a rat liver experiment. KDEL was probed for in total liver tissue, as well as the cytosolic, nuclear, mitochondrial, and ER fractions.
Western Blot
Rat liver tissue
1:250, 4 C, overnight with gentle rocking
1:3 Odyssey block:TBST (0.1% tween)
1:15,000 goat-anti-mouse (Odyssey)
NA
Odyssey with Image Studio software
Really nice blots, minimal background. Observed two really nice bands at the 78 and 94 KDa molecular weights, these correspond to GRP78 and GRP94 retention sequences. There is one additional band that I am getting, I'm assuming it is non-specific banding around 52 KDa. Other than that, clean image and easy to use!
None
Clean image, easy to use
One non-specific band every time
Great antibody, works well, great endoplasmic reticulum marker!!