Good Cytochrome C Antibody

November 05, 2015

Department of Microbiology and Immunology
University of Illinois, Chicago
PostDoctoral Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Cytochrome C (D18C7)

Catalog Number:

11940

I used Cytochrome C antibody to check apoptosis upon treatment in 293T cells. This antibody gave specific bands but requires long exposure.

Experimental Design and Results Summary

Applications

Western Blot

Sample

293T whole cell lysate

Primary Incubation

Anti-cytochrome C diluted in 5% BSA in TBST, incubated O/N at 4 degrees

Blocking Agent

5% milk in TBST

Secondary Incubation

Anti-rabbit HRPO

Tertiary Incubation

NA

Detection

ECL

Results Summary

It gives specific bands at the right size but takes about 10-15 minutes of exposure. Overall, I would score it as a good antibody.

Additional Notes

None

Related Categories

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Summary

The Good

Specificity

The Bad

Long exposure time

The Bottom Line

I would rate it as 4/5.

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