Good Anti-CD8 Antibody for FACS

Medicine
Harvard Medical School
Post-doctoral fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience

Product Name:

Anti-Mouse CD8a FITC

Catalog Number:

11-0081-85

Info: View Product View Product Specs

Browse Similar Products: CD8a Antibodies

I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from the mouse system using anti-mycobacterial ESAT6 binding peptides.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single cell suspensions from mouse lungs and spleen

Primary Incubation

1:100 dilution, after blocking with 2% BSA and Fc block for 1 hour on ice

Blocking Agent

Blocking with 2% BSA and Fc block

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow Cytometry

Results Summary

The ESAT6 binding peptides enhanced the immune response against mycobacterium tuberculosis and resulted in faster clearance of the bacteria from the lungs and spleen. In the presence of the peptide, bacterial antigens were cleared faster.

Additional Notes

None

Related Categories

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Summary

The Good

Good flow cytometry antibody, gives nice signal

The Bad

None

The Bottom Line

Good antibody for surface staining of CD8

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