BD Biosciences
Alexa fluor 647 anti-mouse IFN-gamma
557735
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Browse Similar Products: IFN-gamma Flow Cytometry Antibodies
This antibody binds to mouse interferon gamma, a cytokine that is involved in T cell immunity and inflammation. Since, cytokines have a short half-life and can be consumed during signaling, they are hard to detect. By blocking ER transport for a few hours along with strong re-stimulation, some antibodies can detect IFNg very well. However, this antibody gave a bright fluorescence which was well- separated from the negative population.
Flow Cytometry
Purified mouse T cells
1:200 in Perm/Wash buffer
FcR block
NA
Flow Cytometry - APC channel
The cells show bright IFNg staining which i haven't observed with other fluorophore conjugated antibodies. Background on unstimulated cells is minimal.
None
Bright stain and easy to use
Needs restimulation followed by intracellular staining
It requires a long protocol for staining, but gives good results