T202/Y204-phosphorylated Erk Antibody from Cell Signaling Technology

November 11, 2015

Institute of Genetic Medicine
Johns Hopkins University
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Cell Signaling Technology

Product Name:

T202/Y204-phosphorylated Erk

Catalog Number:

5726

This antibody is used to detect phosphorylation of Erk at T202/Y204 site in response to EGFR inhibitors in human HNSCC FaDu cells.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human HNSCC cell line whole cell lysate

Primary Incubation

1:1000, overnight at 4 degrees

Blocking Agent

5% milk

Secondary Incubation

1:2000, 1 hour at RT

Tertiary Incubation

NA

Detection

Enhanced Chemiluminescence Detection Kit

Results Summary

This antibody clearly detect changes of phosphorylated ERK by western blot.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Detects a specific band and the signal is strong

The Bad

None

The Bottom Line

This is a perfect antibody for western blot.

Share your experience with other scientists. Write a Review! »

Join the discussion