Excellent GAPDH Antibody

Department of Microbiology and Immunology
University of Illinois, Chicago
PostDoctoral Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotechnology

Product Name:

GAPDH Antibody (FL-335) HRP

Catalog Number:

sc-25778 HRP

Browse Similar Products: GAPDH Antibodies

I use GAPDH as a loading control for protein quantification by western blotting. It is one of the best antibodies for western blot. It works every time even at 1:1000 dilution and can be re-used several times.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Whole cell lysate

Primary Incubation

1:10000 diluted in 5% non fat dry milk in TBST, incubated at RT for an Hour

Blocking Agent

5% non fat milk for an hour at RT

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

ECL

Results Summary

This antibody gives single sharp, crisp bands with every single experiment.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Quick and reliable quantification

The Bad

None

The Bottom Line

Awesome antibody for loading control in western blot

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