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PE anti-mouse TNF-α Antibody
506306
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This antibody was used to detect populations of cells producing TNFa after treatment with restimulation cocktail (either PMA, Ionamycin, Bref.A or viral peptide). This antibody was recommended by a collaborating lab.
Flow Cytometry
Primary BMDMs
25 minutes
FC block
NA
LSRII Flow Cytometer
Works well. With our permeabilization kit we were able to detect >60% of CD8+ cells producing TNFa after non-specific restimulation.
None
Works Well
Works great with a reliable permeabilization kit.