BD Biosciences
PE-Cy™7 Rat Anti-Mouse IL-2
560538
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Mouse spleen cells were stimulated (6 hours) with PMA and Ionomycin in presence of protein transport inhibitor Golgi Stop. The cells were first stained with BV510 Rat Anti-Mouse CD4 antibody followed by fixation with BD Cytofix™ Fixation Buffer. The cells were later permeabilized with BD Perm/Wash™ Buffer and stained with and PE-Cy™7 Rat Anti-Mouse IL-2 antibody.
Flow Cytometry
Splenocytes from mice
30 min
NA
Flow cytometry on PE/Cy7 Channel
Presence of IL-2 expressing cells in PMA/Ionomycin treated set confirm the specificity of this antibody.
None
Great specificity
Very good PE-Cy™7 Rat Anti-Mouse IL-2 antibody