Good CD8a Antibody

Department of Microbiology and Immunology
University of Illinois, Chicago
PostDoctoral Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience

Product Name:

Anti-Mouse CD8a eFluor® 450

Catalog Number:

48-0081-80

Info: View Product View Product Specs

Browse Similar Products: CD8a Antibodies

I used mouse thymocytes to stain CD8+ cells in mouse thymocytes. Both CD4 and CD8 are the major markers for TH2 and TH1 cell respectively. My experiment goes very smoothly with antibodies from eBioscience.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse thymocytes

Primary Incubation

0.5 microlitres of antibody for staining 200 000 cells

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry- Cyan

Results Summary

I got specific staining for CD8+ T cells in mouse thymocytes.

Additional Notes

None

Related Categories

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Summary

The Good

Quick and reliable quantification

The Bad

None

The Bottom Line

Nice antibody for flow cytometry. It's fast, specific anf highly efficient.

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