Ponceau S Solution

Miami
MI
Student

Overall

Quality of Results

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Company:

Santa Cruz Biotechnology

Product Name:

Ponceau S Solution

Catalog Number:

sc-301558

Solution is used to detect protein bands on PVDF membranes to determine protein transfer efficiency in western blots. Easy to use solution that can be destained with NaOH.

Experimental Design and Results Summary

Application

Protein Staining

Starting Material

None

Protocol Overview

Add solution to transferred membrane and let stain for a few minutes (typically 5 min.). Determine protein staining and destain with 0.1M NaOH for 1 min.

Tips

Submerge the membrane completely

Results Summary

PVDF membranes show proteins bands after staining and can be easily destained and then blocked and probed for proteins of interest. Good pre-made solution for detecting transferred protein.

Additional Notes

None

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Summary

The Good

Easy to use

The Bad

Costs more than making yourself

The Bottom Line

Nice Ponceau S solution for protein detection.

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