BD Biosciences
V450 Rat Anti-Mouse TNF
560655
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Splenocytes from C57BL/6 mice were stimulated for 6 hrs with PMA and Ionomycin in the presence of protein transport inhibitor Golgi Stop. Cells were harvested and surface stained with BV-510 conjugated rat anti-mouse CD4 antibody. These cells were later fixed and permeabilized using Fixation/Permeabilization Solution Kit followed by staining with rat anti-mouse TNF antibody.
Flow Cytometry
Splenocytes from mice
30 min
NA
Flow cytometry on PB channel
Presence of TNF-positive cells confirm the specificity of this antibody.
None
Very good clear signal and reproducibility
Very good antibody to detect mouse TNF expression