Anti Mouse CD19 FITC for Flow Cytometry

November 06, 2015

BlueskyReddit
Internal Medicine.
Rush University Medical Center
Research Assistant

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Company:

BioLegend

Product Name:

FITC anti-mouse CD19 Antibody

Catalog Number:

115506

Info: View Product View Product Specs

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I used this to identify the CD19+ B Cell population in the mouse spleen to use for downstream research.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spleen

Primary Incubation

1X10^6 suspension cells were incubated with 0.5ul FITC anti-mouse CD19 in 50ul FACS buffer for 30 min at 4oC.

Blocking Agent

0.5ul CD16/CD32 for 10 min at 4oC

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

NA

Results Summary

The graph shows it is not a good Ab to identify B cell population.

Additional Notes

None

Related Categories

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Summary

The Good

NA

The Bad

Not very good.

The Bottom Line

I will not recommend this Ab to other scientists.

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