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Date Published
MIP-CreERT2 Pancreatic Cryosection 1 Day after Tamoxifen Treatment
(1)
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MilliporeSigma
February 18, 2016
Cre transgenes are driven by specific gene regulatory sequences, in our case, under the Mouse Insulin Promoter. The Cre-ERT2 is only ...
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Anti-GFAP as an Astrocyte Marker (for IHC/IF) from EMD Millipore
(1)
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MilliporeSigma
January 21, 2016
This antibody was used in a study that investigated the crossover of astrocytes with aromatase in the teleost brain. It has not yet been ...
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A Great Antibody for Mitosis Marker
(1)
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MilliporeSigma
January 12, 2016
Analysis of mammalian cells in the mitotic phase using flow cytometry is easy and sensitive. The phosphorylated histone 3 at the Ser10 is a ...
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Sox9 Staining in e15.5 Mouse Pancreatic Tissue
(1)
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MilliporeSigma
January 11, 2016
The SRY/HMG box (Sox) family of transcription factors are expressed in early pancreatic development. Among these, Sox9 is expressed at its ...
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Western Blot Using Anti-Phospho-Akt (Ser473), Clone 6F5
(1)
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MilliporeSigma
December 30, 2015
Here, we performed Western blot analyses of phosphorylated Akt1 in cell lysates of human Podocytes using the Anti-phospho-Akt (Ser473), ...
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Western Blot Using Anti-Akt1/PKBa (#07-416)
(1)
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MilliporeSigma
December 29, 2015
Here, we performed Western blot analyses of Akt1/PKBa protein expression in cell lysates of human podocytes using the Akt1/PKBa antibody ...
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Best Phosphatase Inhibitor Cocktail on the Market
(1)
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MilliporeSigma
December 22, 2015
Our lab works with cell lysates to study various proteins related to cancer. Many of these proteins are quickly degraded by phosphatases, ...
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Western Blot Analyses Using Anti-Phospho-mTOR (Ser2448)
(1)
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MilliporeSigma
December 18, 2015
Here we performed Western blot analyses of phospho-mTOR in cell lysates of human podocytes using the anti-phospho-mTOR (Ser2448) antibody.
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A Cheap Primary Antibody With OK Protein Recognization
(1)
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MilliporeSigma
December 18, 2015
This is a cheap primary antibody compared with all other common primary antibodies from rabbit. Dilution is also okay, 1:2000 does the job. ...
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Western Blot Using Anti-Phospho-AMPK1 (Thr174) (#09-290)
(1)
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MilliporeSigma
December 17, 2015
In this experiment we performed Western blot analyses of phosphorylated AMPK1 protein in cell lysates of human Podocytes using the Anti-...
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Western Blot Using Anti-mTOR, Clone F11 (1:500)
(1)
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MilliporeSigma
December 17, 2015
Here, we performed Western blot analyses of mTOR protein expression in cell lysates of human Podocytes using the Anti-mTOR, clone F11 ...
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FACS Staining of RSV Infected PBMCs
(1)
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MilliporeSigma
December 11, 2015
RSV infected PBMCs were detected using this FITC-conjugated monoclonal antibody for extracellular staining.
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An Excellent Alternative to the Mouse anti-NeuN Antibodies
(1)
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MilliporeSigma
December 09, 2015
This antibody works very well and provides a good signal to noise ratio. Its pattern of staining is equivalent to the monoclonal mouse anti ...
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Western Blot Using Anti-AMPK a1 (07-350)
(1)
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MilliporeSigma
December 09, 2015
In this experiment we performed Western blot analyses of AMPKa1 protein expression in cell lysates of human Podocytes using the Anti-AMPK a1...
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Best Phosphatase Inhibitor Cocktail
(1)
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MilliporeSigma
December 03, 2015
Our lab works with cell lysates to study various proteins related to cancer. Many of these proteins are quickly degraded by phosphatases, ...
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Messy Staining for Class-II MHC
(1)
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BioLegend, MilliporeSigma
November 13, 2015
Class-II MHC is expressed on all antigen presenting cells including DCs, macrophages and B cells. The expression goes up during an immune ...
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Good Western Blot Antibody that Works
(1)
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MilliporeSigma
November 11, 2015
This antibody is mouse anti-human fibronectin N-terminal monoclonal antibody. Used in detecting human intervertebral disc tissue. Worked.
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Protease Inhibitor
(1)
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MilliporeSigma
October 19, 2015
This protease inhibitor cocktail is great for using to lyse cells to run western blots; it prevents protein degradation.
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Excellent p300 CT Antibody
(1)
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MilliporeSigma
October 12, 2015
In the present study, mammalian cells were tested for p300 prorein levels in response to treatment. Equal amount of proteins were resolved ...
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Merck Amicon columns to concentrate RNA
(1)
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MilliporeSigma
October 05, 2015
I have used the Merck Millipore columns to concentrate Total RNA. I wanted to maximise RNA recovery by using a number of sample aliquots for...
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Western Blot Analysis of PHB2
(1)
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MilliporeSigma
September 04, 2015
Our laboratory is focused on studying the role of EGFR in de novo fatty acid synthesis and mitochondrial dynamics. We determined the effect ...
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Antibody to Analyze Renilla Luciferase Levels
(1)
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MilliporeSigma
August 17, 2015
We are studying miRNA inhibitors in different cancers by using a dual luciferase system to find better therapeutic small molecules as the ...
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Good RAF1 antibody for Western Blot
(1)
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MilliporeSigma
June 22, 2015
I was looking at RAF1 expression in iPSCs to study RAF1 function in human iPSC-derived cardiomyocytes. I used this product to detect ...
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pCREB expression in Neuronal cells
(1)
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MilliporeSigma
June 15, 2015
We used this product to check the pCREB expression in neuronal cells and its role in neural functioning. Compared to other company's ...
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PD98059 to check MAPK/ERK signaling
(1)
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MilliporeSigma
June 03, 2015
This product was used to check the molecular signaling of neurotrophic compounds. The product inhibits MAK1/MEK2 and is useful for studying ...
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H3 pan as loading control for checking histone modifications
(1)
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MilliporeSigma
June 03, 2015
We used this product to check the histone acetylation in glioblastoma cells treated with anticancer drugs.
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Acetyl H3 expression in GL-261
(1)
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MilliporeSigma
June 02, 2015
We used this product to check the expression of Acetyl H3 in Gl-261 treated with anticancer compounds. This product works relatively well.
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Acetyl histone H4 expression in HDACI treated GL-261 cells
(1)
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MilliporeSigma
June 01, 2015
This product was used to check the differential acetyl H4 expression in hdaci treated Mouse glioma (GL261) cells. This product works well to...
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Good but not great for staining tissues
(1)
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MilliporeSigma
March 27, 2015
Following MPTP, we wanted to quantify the total number of surviving neurons in the SNpc using an antibody specific for MAP2. Fixed brain ...
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Used for evaluation of whole brain homogenate
(1)
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MilliporeSigma
March 24, 2015
This antibody was used to evaluate the effects that MPTP can have on the phosphorylation status of tyrosine hydroxylase at serine19. ...
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Lysyl Oxidase (LOX) Antibody for IHC
(1)
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MilliporeSigma
March 17, 2015
This antibody was used to perform immunohistochemistry in paraffin-embedded tissue sections of mouse mammary gland and human breast tissues....
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NeuN Antibody for Western Blot
(1)
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MilliporeSigma
March 04, 2015
This product was used to monitor expression levels of NeuN upon N2A cell differentiation to study the Differentiation of neuroblstoma (N2A ...
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Tubulin beta-III for Immunocytochemistry
(1)
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MilliporeSigma
March 04, 2015
This product was used to stain the differentiated neuroblastoma cells (N2A cells) to study the differential neurite out growth after ...
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Good antibody for immunohistochemistry and immunoblotting
(1)
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MilliporeSigma
February 23, 2015
I used this antibody for immunoblotting and immunohistochemistry on brain tissue samples. The antibody worked well for both techniques and ...
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Anti-tyrosine hydroxylase antibody for fresh frozen tissue
(1)
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MilliporeSigma
February 20, 2015
This antibody is used to look for dopaminergic neurons within the SNpc and their termini within the striatum using the MPTP-mouse model of ...
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Good loading control for immunoblotting
(1)
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MilliporeSigma
February 20, 2015
For immunoblotting, a good loading control is always as important as proteins that we want to analyze. GAPDH, tubulin, and actin are the ...
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Anti-Tyrosine Hydroxylase Rabbit pAb for neuronal staining
(1)
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MilliporeSigma
February 18, 2015
The aim of my research is to determine TH+ neuron counts within the substantia nigra following MPTP exposure and drug treatment. I used this...
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Anti-Fibroblasts, Clone TE-7 Does Label Fibroblastic Cells
(1)
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MilliporeSigma
February 13, 2015
We wanted to label fibroblastic cells in the gastrocnemius of Peripheral Artery Disease patients to establish the presence of progressive ...
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GAD67 Antibody Works Well for Immunohistochemistry
(1)
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MilliporeSigma
February 12, 2015
I wanted to determine GAD67 location in fixed brain tissue. This antibody had been left in the refrigerator for years before I used it. It...
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Great antibody for immunohistochemistry, lasts a long time
(1)
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MilliporeSigma
February 04, 2015
I wanted to detect calretinin in fixed brain tissue via immunohistochemistry. I pulled this antibody out of a -20 degrees C freezer and ...
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CFTR antibody for Western blot from EMD Milllipore
(1)
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MilliporeSigma
December 26, 2014
This antibody was used in investigation of regulation of the cystic fibrosis transmembrane conductance regulator (CFTR). Several antibodies ...
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Easy-to-use kit for studying RBP-RNA interactions
(1)
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MilliporeSigma
December 23, 2014
One of the goals of my research is to identify proteins that interact with a specific mRNA. We had previously identified candidate proteins ...
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H2AX pS139 in response to CPT
(1)
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MilliporeSigma
December 17, 2014
H2AX is a histone variant that is required for DNA damage response. Phosphorylation of H2AX on S139 can be induced by ionizing radiation and...
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Good early stem cell marker
(1)
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MilliporeSigma
December 05, 2014
These antibodies were used in work in my previous lab with Dr. Scott Whittemore at the Kentucky Spinal Cord Injury Research Center at the ...
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Failed to improve GFP signal
(1)
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MilliporeSigma
November 26, 2014
Embryonic rat dorsal root ganglion neurons were infected with a GFP-tagged lentivirus in culture. The signal from the virus was present, ...
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P38 protein expression was characterized via Western blotting using the Anti-p38 MAP Kinase (341-360) Rabbit pAb
(1)
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MilliporeSigma
November 21, 2014
P38 protein expression was characterized via Western blotting using the Anti-p38 MAP Kinase (341-360) Rabbit pAb (1:1000) in HL-1 cells
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GAPDH protein expression analysis via Western blotting
(1)
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MilliporeSigma
November 07, 2014
GAPDH protein expression was characterized via Western blot using the anti-GAPDH antibody (1:10000).
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FGF2 protein expression via western blotting
(1)
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MilliporeSigma
October 27, 2014
FGF2 protein expression was characterized via western blot using the Anti-FGF-2/bFGF Antibody (1:500) in murine HL-1 cells.
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The Usage of PP2Ac ab
(1)
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MilliporeSigma
August 06, 2014
To detect the protein abundance of PP2Ac in arabidopsis with this PP2Ac antibody. This antibody uses the most conserved sequence of PP2Ac ...
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S100A4 Detected by IHC
(1)
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MilliporeSigma
July 07, 2014
We use this marker to detect those active fibroblasts in tumoral samples to analyze patient or mice tumoral samples.
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