Calbiochem
Anti-Tyrosine Hydroxylase Rabbit pAb
657012
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The aim of my research is to determine TH+ neuron counts within the substantia nigra following MPTP exposure and drug treatment. I used this product as a primary antibody to the Tyrosine hydroxylase protein within dopaminergic neurons. I chose this product because another lab in our program had successfully used it with reliable results.
Immunohistochemistry
Midbrain sections
1:2000 in 0.1M TBS with 2% NGS, 48 hours, 4C
0.1M TBS with 2% NGS
Goat anti-rabbit Ig 1:400 in 0.1 M with 2% NGS, 60 minutes, room temperature
N/A
DAB peroxidase color development
I was able to successfully stain dopaminergic cell bodies within the substantia nigra along with termini in the striatum. The results are consistent and reliable, and there was no non-specific staining seen.
None
Easy to use and good staining
I don't feel that there is a drawback to using this product.
The product was reliable, stained evenly and where expected, and had no non-specific binding.