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Select Type
Antibodies
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Date Published
Further Optimizations Needed for Anti-Aquaporin 4 Antibody
(1)
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MilliporeSigma
August 12, 2020
This antibody was used to detect the astrocytic end feet, positive for Aquaporin4, which surround the blood vessels.
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Good Results With Monoclonal Anti-ß-Tubulin Isotype III from Sigma
(1)
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MilliporeSigma
August 05, 2020
The antibody also works well with thick vibratome sections up to 250 µm as the penetration of the antibody is good.
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Purification of IgG from Rabbit antisera
(1)
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MilliporeSigma
July 22, 2020
Highly recommended for purification or separation of IgG or IgG subclass from the samples.
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Tryspin for Tissue Dissociation
(1)
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MilliporeSigma, Merck
July 13, 2020
Highly recommended for primary neuronal cultures from hippocampus and cortex.
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GAPDH Activity Assay Kit Used for Cell Culture Gives Reliable Results
(1)
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MilliporeSigma
July 04, 2020
I use this GAPDH Activity Assay Kit to measure the enzyme activity of glyceraldehyde-3-phosphate dehydrogenase in cell culture samples.
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FACS Sorting of Wildtype-EGFR Expressing Glioma Cells using Anti-EGFR Antibody
(1)
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MilliporeSigma
June 29, 2020
Excellent antibody against wildtype-EGFR for cell sorting.
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Anti-ATM Antibody that Works for Immunoblotting
(1)
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MilliporeSigma
June 29, 2020
I used this antibody to detect ATM levels in various cell lines using Immunoblotting.
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Mouse IgG HRP Antibody Is Great for Western Blotting
(1)
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MilliporeSigma
June 26, 2020
This is a highly recommended secondary antibody for western blots.
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Highly Versatile and Efficient Taq DNA
(1)
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MilliporeSigma
June 26, 2020
I will definitely suggest this Taq DNA polymerase if you are you looking for an efficient Taq with a good processivity for cloning and ...
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Anti-Actin Antibody that Works Great for Immunoblotting
(1)
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MilliporeSigma
June 22, 2020
I have been using this antibody for 5 years to detect b-Actin levels in various cell lines using Immunoblotting.
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Good Antibody for Phosphotyrosine from MilliporeSigma
(1)
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MilliporeSigma
June 19, 2020
Not sure if this antibody works well for cell lysate. But it works pretty well for purified protein installed with phosphorylated tyrosine.
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Robust Reverse Transcriptase
(1)
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MilliporeSigma
June 17, 2020
I strongly recommend this enzyme if you are working with a low copy number gene expression.
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Steriflip Makes Filtration of Media and Other Reagents Easy
(1)
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MilliporeSigma
June 16, 2020
If sterilization of cell culture media and reagents is needed in smaller amounts than the 250 or 500 mL filter units, the Steriflip is a ...
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A Starvation Medium for Imaging Analysis and Autophagy Study
(1)
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MilliporeSigma
June 09, 2020
This media induces autophagy within 30 minutes.
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High Quality TEV Protease for Protein Purification
(1)
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MilliporeSigma
June 08, 2020
I would recommend this TEV protease for cleaving TEV specific sites. It could be purified by yourself if you want to save money.
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Good Astrocyte Endfeet Marker
(1)
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MilliporeSigma
June 08, 2020
We regularly use this antibody to label astrocyte endfeet. Works really well.
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Very Good Tubulin Antibody from MilliporeSigma
(1)
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MilliporeSigma
June 05, 2020
Very specific antibody.
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Deoxyribonuclease I Is Ideal for Digesting DNA to Remove Interference
(1)
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MilliporeSigma
June 03, 2020
DNase I from bovine pancreas is an effective enzyme for digesting DNA to help clear it and prevent obstruction of downstream analyses. It ...
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Water Purification System for Molecular Biology Experiment
(1)
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MilliporeSigma
June 03, 2020
Although expensive, a biology lab must have one.
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In Situ Cell Death Detection Kit
(1)
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MilliporeSigma
June 02, 2020
The kit should be used to analyze apoptosis following the classical method of DNA strand breaking. Microscopy enables very convenient ...
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Large Volume of Anti-Flag Magnetic Beads for Mass-Spec Experiment
(1)
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MilliporeSigma
June 01, 2020
If you are performing anti-flag immunoprecipitation often, this is very economic and convenient reagent that the entire laboratory can share...
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Great Solution for Density Gradient Separation
(1)
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MilliporeSigma
May 28, 2020
Great ready to use solution to process whole PBMCs to obtain leukocytes and plasma.
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Great Trypsination Solution to Detach Cells
(1)
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MilliporeSigma
May 27, 2020
Great solution to trypsinize adherent cells from plastic.
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Hydrophobic PVDF Transfer Membrane with Low Background Fluorescence for Western Blotting
(1)
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MilliporeSigma
May 20, 2020
I would keep one roll of this product for the lab.
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cOmplete™, Mini Protease Inhibitor Cocktail Is Useful for Preparing Protein Lysates for Western Blotting
(1)
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MilliporeSigma
May 20, 2020
The protease inhibitor cocktail is very easy to use; although according to the company it appears not to be stable when frozen at -20 ...
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Donkey Serum Is Great for Blocking Specimens for Secondary Antibodies for IHC
(1)
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MilliporeSigma
May 18, 2020
Donkey serum should be used to block non-specific antigens for IHC when incubated specimens with donkey secondary antibodies. This serum is ...
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Perfect Antibody for FLAG Tag
(1)
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MilliporeSigma
May 12, 2020
This is one of the best antibodies with high specificity and high affinity. It works very well for proteins with FLAG tag.
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Works Great to Induce Inflammation
(1)
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MilliporeSigma
May 11, 2020
This works well to induce an inflammation in the hind paw of mice.
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This Is a Sensitive Antibody against Puromycin to Roughly Check Protein Translation Rate
(1)
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MilliporeSigma
May 11, 2020
This reagent will tell you the translational level change.
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Must-Have Membrane in a Biological Lab
(1)
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MilliporeSigma
May 07, 2020
This membrane works well for most western blots and is compatible with over 100 antibodies that I have tried so far.
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PVDF Membrane for Small Molecular Weight Protein Detection
(1)
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MilliporeSigma
May 06, 2020
I would try this with a small samples, and if the trial works, then I'd order a whole box of the membrane.
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Works at 1:100 to Detect CK2 Complexes, Mostly Nuclear Localized
(1)
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MilliporeSigma
April 28, 2020
This was an efficient antibody that yielded publishable results by immunofluorescence. Other applications were not tried.
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Good RIPA Buffer
(1)
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MilliporeSigma
April 22, 2020
Recommended for lab routine protein lysis.
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The TOSOH Column Is Useful for Analytical SEC in High Throughput
(1)
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MilliporeSigma
April 22, 2020
The column is used to determine quality of produced proteins by size exclusion chromatography.
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Good Cresyl Violet Stain
(1)
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MilliporeSigma
April 20, 2020
We routinely use for staining of nuclei and Nissl bodies.
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The Superdex 200 100mL Is a High-Performing Preparative Size-Exclusion Column
(1)
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MilliporeSigma
April 20, 2020
If proteins need to be remediated the Superdex column can remove unwanted aggregate or other species from your sample.
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Must-Have Enzyme for Western Blotting
(1)
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MilliporeSigma
April 17, 2020
I highly recommend this enzyme for everybody doing WB!
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Good Substrate for Measuring Dipeptidyl Peptidase-IV Activity in Rat Plasma
(1)
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MilliporeSigma
April 17, 2020
GLY-Pro p-nitroanilide hydrochloride is a good substrate for measuring the activity of dipeptidyl peptidase-IV in plasma.
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Very Effective for Removing RNase
(1)
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MilliporeSigma
April 16, 2020
Necessary for experiments dealing with RNA.
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Good FLAG Antibody from MilliporeSigma
(1)
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MilliporeSigma
April 08, 2020
Definitely recommended for flag detection.
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DMSO for Tissue Culture
(1)
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MilliporeSigma
April 07, 2020
I recommend this DMSO solvent!
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Competent E. Coli Cells for Protein Expression
(1)
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MilliporeSigma
April 07, 2020
I would recommend using this competent cell to express and purify recombinant proteins.
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Very Good Plasmid Midiprep Kit
(1)
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MilliporeSigma
April 07, 2020
Recommended for plasmid extraction.
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Expression of Anti-Mn-SOD in Disease Condition
(1)
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MilliporeSigma
April 03, 2020
This antibody is working good. Highly recommended.
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Poly-L-Lysine to Coat a Glass-Bottom Flask
(1)
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MilliporeSigma
April 01, 2020
I recommend this for live-cell imaging analysis.
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ß-Actin as a Loading Control for Immunoblotting
(1)
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MilliporeSigma
March 30, 2020
A very good loading control for western blot experiments.
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High-Quality Ultrapure Water for Biological Experiments
(1)
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MilliporeSigma
March 27, 2020
This water purification system works well for common lab use.
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ß-Tubulin Antibody Used as a Loading Control for Immunoblotting
(1)
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MilliporeSigma
March 27, 2020
Excellent loading control for immunoblotting experiments.
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Trypsin for Tissue Culture
(1)
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MilliporeSigma
March 26, 2020
Must-have item in a tissue culture room.
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Staining of Primary Murine Microglia Cells
(1)
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MilliporeSigma
March 26, 2020
Excellent staining for visualization of microglia.
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Transfection
Electrophoresis
RT-qPCR
Cell Counting
Protein Purification
Next Gen Sequencing (NGS)
Library Construction
Genotyping
Gel Imaging
Protein Assay
Reporter Assay
Cell Lysis
Multiplex
Gel Staining
RNA-Seq
Live Cell Imaging
Cell Differentiation
Ligation
Concentration
HPLC
Sequencing
Chromatography
Mass Spectrometry
Labeling
Microarray
Mutagenesis
FISH
CRISPR
TUNEL Assay
In Vivo Imaging
EMSA
Cell Fractionation
Immunoassay
In Vitro Transcription
ChIP-Seq
Northern Blot
3D Imaging
RNAi
ELISPOT
PCR Cloning
Particle Analysis
Southern Blot
Protein Modification
DNA Analysis
Expression
Cell-Based Assay
Gene Editing
In Vivo Testing
Protein Labeling
Other
Species
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Human
Mouse
Rat
Non-Human Primate
Rabbit
Sheep
Hamster
Chicken
Bovine
Canine
Porcine
Horse
Monkey
C. elegans/Worm
Insect
Amoeba/Protozoa
Bacteria/Archaea
Arabidopsis
Yeast/Fungi
Squirrel
Fish
Virus
Other Invertebrate
Xenopus/Amphibian
Drosophila
Goat
Rhesus
Tissue
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Adipose
Aorta
Artery
Bladder
Blood
Blood Vessel
Bone
Bone Marrow
Brain
Breast
Bronchus
Cerebral Cortex
Colon
Cornea
Coronary Artery
Dermis
Duodenum
Ear
Embryo
Endometrium
Epidermis
Eye
Foreskin
Gingiva
Hair
Heart
Hippocampus
Hypothalamus
In Vivo
Intestine
Kidney
Large Intestine
Liver
Lung
Lymph Node
Mammary Gland
Mouth
Muscle
Ovary
Pancreas
Peripheral Blood
Peritoneum
Placenta
Prostate
Rectum
Retina
Skeletal Muscle
Skin
Small Intestine
Spinal Cord
Spleen
Stomach
Striatum
Synovium
Tail
Teeth
Testis
Thymus
Thyroid Gland
Tonsil
Trachea
Umbilical Cord
Umbilical Vein
Uterus
Vein
Cell Type
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Adipocyte
Astrocyte
B Cell
CD8+ T Cell
Competent Cells
Dendritic Cell
Embryonic Stem Cell
Endothelial Cell
Eosinophil
Epithelial Cell
Fibroblast
Glial Cell
Granulocyte
Helper T Cell
Hematopoietic Stem Cell
Hepatocyte
HUVEC
Induced Pluripotent Stem Cell
Keratinocyte
Leukocytes
Lymphocyte
Macrophage
Mesenchymal Stem Cell
Microglia
Microvascular Endothelial Cell
Monocyte
Myoblasts
Myocyte
Neuron
Neutrophil
NK Cell
NKT Cell
Oligodendrocytes
Osteoclast
PBMC
Platelets
Progenitor Cell
Red Blood Cell
Regulatory T Cells
Skeletal Muscle Cell
Smooth Muscle Cell
Splenocyte
Stem Cell
Stromal Cell
Synoviocyte
T Cell
Thymocytes
Tumor Cells
Cell Line
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HeLa
PC3 (PC-3)
HEK293
HEK293T (HEK-293T)
CHO
Jurkat
3T3
Caco-2
HepG2
THP-1
U2OS
PC12 (PC-12)
HL60 (HL-60)
A375 (A-375)
MOLT4 (MOLT-4)
MCF-7
A431
MDA-MB-231
A549
N2a
Daudi
HCT-116
Raji
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