Failed to improve GFP signal

November 26, 2014

Cardiovascular Innovation Institute
University of Louisville
Research Coordinator

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Quality of Results

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Company:

EMD Millipore

Product Name:

Polyclonal chicken anti-GFP

Catalog Number:

AB16901

Info: View Product View Product Specs

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Embryonic rat dorsal root ganglion neurons were infected with a GFP-tagged lentivirus in culture. The signal from the virus was present, but weak. Attempts to improve GFP signal with this antibody failed.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Embryonic rat DRG neurons in culture

Primary Incubation

1:500 overnight at 4C

Blocking Agent

PBS + 0.5% BSA + 10% NDS + 0.1% Triton

Secondary Incubation

Donkey anti-Chicken FITC at 1:100 for 1 hour at room temperature, protected from light

Tertiary Incubation

N/A

Detection

Widefield fluorescence microscopy

Results Summary

Embryonic rat dorsal root ganglion neurons were infected with a GFP-tagged lentivirus in culture. The signal from the virus was present, but weak. Attempts to improve GFP signal with this antibody failed.

Additional Notes

NA

Related Categories

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Summary

The Good

Somewhat specific

The Bad

Very faint, failed to improve GFP signal

The Bottom Line

Would not recommend

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