EMD Millipore
Anti-Sox9 Antibody
AB5535
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The SRY/HMG box (Sox) family of transcription factors are expressed in early pancreatic development. Among these, Sox9 is expressed at its highest level in the early pancreatic epithelium. It has been shown that all pancreatic cells derive from Sox9 expressing precursor cells. Adult pancreatic ductal epithelium expresses Sox9.
Immunofluorescence
e15.5 mouse pancreas region; paraffin embedded
1:500; 4 deg C; Overnight
5% Normal Donkey Serum in 1%BSA/PBS (w/v)
1:1000. rabbit IgG-Cy2. 2 hours, Room temperature
NA
Fluorescence microscopy
Sox9 is easily detected in the pancreatic epithelium at e15.5. The counter-stain of Pdx1, a pancreas-specific transcription factor, was used to confirm the presence of pancreatic epithelium region.
Antigen retrieval (TEG, pH9.0) was necessary to detect Sox9 and Pdx1 in paraffin embedded sections.
Excellent detection at 1:500
None
This Sox9 antibody works excellently following antigen retrieval in e15.5 paraffin embedded sections with little background.