Merck Millipore
Anti-FGF-2/bFGF
MABS72
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FGF2 protein expression was characterized via western blot using the Anti-FGF-2/bFGF Antibody (1:500) in murine HL-1 cells.
Western blot
Lysate of murine HL-1 cells
1:500; overnight , 4°C , in TBS-T; 1%BSA; 0.02 Na3
5% low-fat milk solution
1:5000, 1 h, room temperature, rabbit-anti-mouse IgG – HRP conjugated
ECL Plus Detection Reagents (Amersham)
Using the above mentioned antibody (1:500), several bands were detected including a FBF2 band at approximately 17 kDa.
NA
FGF2 was detected by this antibody (17kDa).
Some other bands were detected by this antibody in higher ranges.
This antibody is sufficient for protein expression analyses via Western blotting.