The Good
High specificity and relatively high dilution
The Bad
Requires overnight incubation instead of 1h RT in order to visualize processes, could not validate that the antibody did not stain ependymal cells
The Bottom Line
This is a very good antibody to detect GFAP/astrocytes, and it seem to work very well in the telost brain, of which many antibody are not readily available. That being said, this antibody may not work in other teleost species (we use A. ocellaris), and the protocol may not cross over to other species. Remember to always optimize to your specific needs.