Chemicon
Mouse anti-human fibronectin N-terminal monoclonal antibody
MAB1936
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This antibody is a mouse anti-human fibronectin N-terminal monoclonal antibody. This was used in detecting human intervertebral disc tissue and it worked.
Western Blot
Human disc tissue lysate
1:5000 dilution. Incubate at 4C overnight.
50% of Licor blocking buffer mixed with 50% PBS.
Anti-mouse antibody (1:10,000 dilution,800CW. Li-Cor)
NA
Li-Cor Oddyssey machine at 800cw
This antibody worked with the lysates from human intervertebral disc tissue in western blot assay.
None
Non-specific cross reaction is relatively low
Not found yet
The antibody worked in detecting the N-terminal fibronectin fragment in human disc tissue lysate.