Merck Millipore
Anti-phospho-AMPK1 (Thr174)
09-290
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We performed protein expression analyses in human podocytes regarding the generation of different factors involved in apoptosis regulation, signaling, cell structure maintenance and cell viability. Specifically phospho-specific antibodies were used to analyse the signaling activity normalized againt total protein expression (see Akt, AMPK and mTOR). In this experiment we performed Western blot analyses of phosphorylated AMPK1 protein in cell lysates of human Podocytes using the Anti-phospho-AMPK1 (Thr174) (#09-290) antibody
Western Blot
Cell lysate of human podocytes
Dilution 1:500, 16 hours, 4°C
5% milk powder in TBST buffer
Dilution: 1:5000, 1 hour, room temperature
ECL - 5 min, room temperature
ECL detection
Two bands including one for phospho-AMPK1 (63 kDa) was detected after 5 min (see fig.)
None
Phospho-AMPK1 (63 kDa) was detected
A second band was detected using this antibody
This antibody is okay for Western blot analyses of phospho-AMPK in human podocytes