Merck Millipore
Anti-phospho-Akt (Ser473), clone 6F5
05-1003
Info: View Product View Product Specs
Browse Similar Products: Phospho-Akt Western blot Antibodies
We performed protein expression analyses in human podocytes regarding the generation of different factors involved in apoptosis regulation, signaling, cell structure maintenance and cell viability. Specifically phospho-specific antibodies were used to analyse the signaling activity normalized againt total protein expression (see Akt, AMPK and mTOR). Here, we performed Western blot analyses of phosphorylated Akt1 in cell lysates of human Podocytes using the Anti-phospho-Akt (Ser473), clone 6F5.
Western Blot
Cell lysate of human podocytes
Dilution 1:500, overnight, 4°C
5% Skim milk in TBST
Dilution: 1:5000, 1 hour, room temperature
ECL - 5 min, room temperature
ECL detection
A band including phospho-Akt/PKBa (60 kDa) was detected after 5 min
None
Phospho-Akt/PKBa was detected at 60 kDa, well suited for WB
This antibody is well suited for Western blot analyses of phosphorylated Akt in human podocytes