EMD Millipore
PHB2 antibody
AB10198
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Our laboratory is focused on studying the role of EGFR in de novo fatty acid synthesis and mitochondrial dynamics. We determined the effect of EGF on protein levels of PHB2 in PC3 cells.
Western Blot
PC3 cell lysates
1:2000, overnight at 4 C
5% milk
1:3000, one hour at room temperature
NA
ECL and film
This antibody worked really well for western blot analysis and also can be used as a mitochondrial marker protein
None
Very easy to detect PHB2 with this antibody compared to the other sources
Not very good for immunocytochemistry
Works well at least for western blot