Merck Millipore
Anti-acetyl-Histone H3 Antibody
06-599
Info: View Product View Product Specs
Browse Similar Products: Acetyl-Histone H3 Antibodies
We do work on gene and protein expression changes (epigenetic modulators) in neuroglia differentiation and behaviour. We used this product to check the expression of Acetyl H3 in Gl-261 treated with anticancer compounds. This product works relatively well.
Immunofluorescence
Gl-261 cells
1:500 in 2% BSA overnight 4 C
2% BSA iN 1xPBST(tritonX-100)
Rabbit-Alexa488 1:400, Room temperature, 90 min
No tertiary incubation
Flourescent microscope
In HDACi treated cells the Acetyl H3 levels are increased compared to control.
Coverslip fixed on slide with vectorshield mixed with DAPI
Works well
Cost
Recommended for use for checking the acetyl H3 levels in cells.