EMD Millipore
Anti-phospho-CREB (Ser133) Antibody
06-519
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Our work focuses on gene and protein changes (epigenetic modulators) involved in neurotrophic and neuroprotective activity. We used this product to check the pCREB expression in neuronal cells and its role in neural functioning. Compared to other company's products, this product works well.
Western Blot
Neuroblastoma (N2A) cells
1:1000 in 5%BSA(1XPBST), Overnight, 4C
5%BSA in 1xTBST(0.1%TritonX100)
1:5000, anti-rabbit-HRP, 2h, Room temperature
NA
Chem-doc was used to detect the chemiluminiscence
pCREB expression changes in neurotrophicity.
None
Good signal
Non-specific band near the band of interest
Can use to study the role of pCREB in neural functioning