EMD Millipore
Anti-acetyl-Histone H4
06-866
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We do work on gene and protein expression changes (epigenetic modulators) in neuroglia differentiation and behaviour. This product was used to check the differential acetyl H4 expression in hdaci treated Mouse glioma (GL261) cells. This product works well to check H4 acetylation.
Western Blot
Cell lysate of GL261
1:500, overnigh 4 C
5%BSA in 1xTBST(0.1%TritonX100)
Anti-rabbit-HRP, 2h, Room temperature
No tertiary incubation
Chem-doc was used to detect the chemiluminiscence
In HDACi treated samples the acetylation of H4 is high compared to control.
15% gel
Specificity
Less dilution(1:500), band comes at ~11kDa
Recommended to use