EMD Millipore
Monoclonal Mouse anti-Nestin
MAB353
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These antibodies were used in work in my previous lab with Dr. Scott Whittemore at the Kentucky Spinal Cord Injury Research Center at the University of Louisville. We were characterizing cells from our then-novel technique of isolating mouse oligodendrocyte precursor cells from the cortex of post-natal mice. Since this line of cells is a progenitor line, we used quite a few different antibodies to convince ourselves that we had the cells we were looking for, at the right developmental stage. Some of the antibodies were used to show that the cells were expressing appropriate markers; others were used to insure that we did not have inappropriate markers in our cell line, and thus in impure population. Once we were confident in the cell line, we then co-cultured them with rat dorsal root ganglion neurons as an in vitro model of myelination. This antibody works well.
Immunofluorescence
Late passage mouse oligodendrocyte precursor cells.
1:200 overnight at 4C.
PBS + 0.5% BSA + 10% NDS + 0.1% Triton
Donkey anti-Mouse FITC at 1:100 for 1 hour at room temperature.
N/A
Widefield fluorescence microscopy
After several passages, mouse oligodendrocyte precursor cells lose their O4 specificity and "de-differentiate" and begin to express earlier stem cell markers such as nestin. This antibody worked well to show these nestin+ cells.
Specific
NA
Nice antibody