P38 protein expression was characterized via Western blotting using the Anti-p38 MAP Kinase (341-360) Rabbit pAb

November 21, 2014

Kli.Pha.-Tox
Charité - Universitatsmedizin Berlin, CCM
Ph.D.

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review
P38 protein expression identified using anti-p38 MAP Kinase antibody via Western blot.

Company:

Merck Millipore

Product Name:

Anti-p38 MAP Kinase (341-360) Rabbit pAb

Catalog Number:

506123-200UL

P38 protein expression was characterized via Western blotting using the Anti-p38 MAP Kinase (341-360) Rabbit pAb (1:1000) in HL-1 cells.

Experimental Design and Results Summary

Applications

Western blot

Sample

Cell lysate of murine HL-1 cells

Primary Incubation

1:1000; overnight , 4°C , in TBS-T; 1%BSA; 0.02 Na3

Blocking Agent

5% low-fat milk solution

Secondary Incubation

1:5000, 1h, room temperature, goat-anti-rabbit IgG – HRP conjugated

Tertiary Incubation

5% low-fat milk solution

Detection

ECL Plus Detection Reagents from Amersham

Results Summary

Using the above mentioned antibody (1:1000), one distinct band was detected for p38 at 38 kDa.

Additional Notes

NA

Related Categories

Image Gallery

P38 protein expression identified using anti-p38 MAP Kinase antibody via Western blot.

Summary

The Good

One distinct band was detected for p38 at 38 kDa.

The Bad

None

The Bottom Line

This antibody worked well in Western blotting.

Share your experience with other scientists. Write a Review! »

Join the discussion