Merck Millipore
Anti-AMPK α1
07-350
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We performed protein expression analyses in human podocytes regarding the generation of different factors involved in apoptosis regulation, signaling, cell structure maintenance and cell viability. Specifically phospho-specific antibodies were used to analyse the signaling activity normalized againt total protein expression (see Akt, AMPK and mTOR). In this experiment we performed Western blot analyses of AMPKa1 protein expression in cell lysates of human Podocytes using the Anti-AMPK α1 antibody from Merck Millipore
Western Blot
Cell lysate of human podocytes
Dilution 1:500, 16 hours, 4°C
5% Skim milk in TBST
Dilution: 1:5000, 1 hour, room temperature
ECL - 5 min, room temperature
ECL
A single band was detected after 5 min (AMPKa1-band at 63 kDa; see figure).
None
Single band was detected after 5 min (AMPKa1-band at 63 kDa; see figure), well suited for WB
This antibody is well suited for Western blot analyses of AMPKa1 expression in human podocytes