Merck Millipore
Anti-phospho-mTOR (Ser2448)
09-213
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We performed protein expression analyses in human podocytes regarding the generation of different factors involved in apoptosis regulation, signaling, cell structure maintenance and cell viability. Specifically phospho-specific antibodies were used to analyse the signaling activity normalized againt total protein expression (see Akt, AMPK and mTOR). Here we performed Western blot analyses of phospho-mTOR in cell lysates of human podocytes using the anti-phospho-mTOR (Ser2448) antibody.
Western Blot
Cell lysate of human podocytes
Dilution 1:500, 16 hours, 4°C
5% milk powder in TBST buffer
Dilution: 1:5000, 1 hour, room temperature
ECL - 5 min, room temperature
ECL detection
Several bands were detected after 5 min including a phospho-mTOR band at 289 kDa (see figure).
phospho-mTOR was detected at 289 kDa
several other bands were detected
This antibody is ok for Western blot analyses of phospho-mTOR in cell lysates of human podocytes