GAPDH protein expression analysis via Western blotting

November 07, 2014

Kli.Pha.-Tox
Charité - Universitatsmedizin Berlin, CCM
Ph.D.

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GAPDH protein identified using Anti-GAPDH antibody via Western blot.

Company:

Merck Millipore

Product Name:

Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5

Catalog Number:

MAB374

Info: View Product View Product Specs

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GAPDH protein expression was characterized via Western blot using the anti-GAPDH antibody (1:10000).

Experimental Design and Results Summary

Applications

Western blot

Sample

Lysate of human endothelial cells

Primary Incubation

1:10000; overnight , 4°C , in TBS-T; 1%BSA; 0.02 Na3

Blocking Agent

5% low-fat milk solution

Secondary Incubation

1:10000, 1 h, room temperature, rabbit-anti-mouse IgG – HRP conjugated

Tertiary Incubation

5% low-fat milk solution

Detection

ECL detection

Results Summary

Using the above mentioned antibody (1:10000) one distinct band was detected for GAPDH at 36 kDa.

Additional Notes

NA

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GAPDH protein identified using Anti-GAPDH antibody via Western blot.

Summary

The Good

One distinct band was detected for GAPDH at 36 kDa; low amounts were needed for detection.

The Bad

None

The Bottom Line

One distinct band was detected for GAPDH at 36 kDa; low amounts were needed for detection. This antibody works well for Western blotting.

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