Merck Millipore
Anti-Akt1/PKBα
07-416
Info: View Product View Product Specs
Browse Similar Products: Akt1/PKBalpha Western blot Antibodies
We performed protein expression analyses in human podocytes regarding the generation of different factors involved in apoptosis regulation, signaling, cell structure maintenance and cell viability. Specifically phospho-specific antibodies were used to analyse the signaling activity normalized againt total protein expression (see Akt, AMPK and mTOR). Here, we performed Western blot analyses of Akt1/PKBα protein expression in cell lysates of human podocytes using the Akt1/PKBα antibody from Merck Millipore
Western Blot
Cell lysate of human podocytes
Dilution 1:500, 16 hours, 4°C
5% milk powder in TBST buffer
Dilution: 1:5000, 1 hour at room temperature
ECL - 5 min, room temperature
ECL
A band including Akt/PKBa (60 kDa) was detected after 5 min
None
Akt/PKBa (60 kDa) was detected, Ab`s easy to use
This antibody is well suited for Western blot analyses of Akt expression in human podocytes